AFDye 488 TFP Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1428-100μg
3 x 100 μg
$129.00
In stock
1428-1
1 mg
$139.00
In stock
1428-5
5 mg
$419.00
In stock
1428-25
25 mg
$1,495.00
In stock

AFDye™ 488 TFP Ester (Alexa Fluor® 488 TFP Ester equivalent) is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AFDye™ 488 conjugates are highly photostable dyes with very high fluorescence quantum yields.

Abs/Em Maxima
494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight
682.53 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>90% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 488 TFP Ester (Alexa Fluor® 488 TFP Ester equivalent) is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AFDye™ ideally suited for the 488 nm line. AFDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AFDye™ 488 TFP Ester can be used a less expensive alternative to Alexa Fluor® 488 NHS Ester.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

AFDye488

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