AZDye 488 TFP Ester

1428-1

1 mg

$189.00

In stock

1428-5

5 mg

$565.00

In stock

1428-25

25 mg

$1,850.00

In stock

AZDye™ 488 TFP Ester is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AZDye™ 488 conjugates are highly photostable dyes with very high fluorescence quantum yields.

AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.

DyLight® and Alexa Fluor® are registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima

494/517 nm

Extinction Coefficient

73,000

Flow Cytometry Laser Line

488 nm

Microscopy Laser Line

488 nm

Spectrally Similar Dyes

FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488

Molecular weight

682.53 (protonated)

CAS

N/A

Solubility

Water, DMSO, DMF

Purity

>90% (HPLC)

Appearance

Orange to light red solid

Storage conditions

-20°C. Desiccate

Shipping conditions

Ambient temperature

AZDye™ 488 TFP Ester is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AZDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AZDye™ ideally suited for the 488 nm line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

AZDye488

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *

* Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)