AZDye 488 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1276-1
1 mg
$190.00
In stock
1276-5
5 mg
$565.00
In stock
1276-25
25 mg
$1,795.00
In stock

    AZDye™ 488 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Learn more about picolyl azide reagents.
    Abs/Em Maxima
    494/517 nm
    Extinction Coefficient
    73.000
    Spectrally Similar Dyes
    Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488
    Molecular weight
    736.69 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Yellow solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye™ 488 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A.

    DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    AZDye488

    1. Ratnayeke, N., et al. (2022). CDC7-independent G1/S transition revealed by targeted protein degradation. Nature., 605 (7909), 357-365. [PubMed]
    2. Ratnayeke, N., et al. (2021). Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. bioRxiv, e-print. [bioRxiv]
    3. Köberlin, M. S., et al. (2021). LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol., 220 (8), e202009147. [PubMed]
    4. Morral, C., et al. (2020). Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro. STAR Protocols, Volume 1, 2 [ScienceDirect]
    5. Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry, 10, 1093. [Oxford Academic]
    6. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
    7. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
    8. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
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