AFDye 488 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1338-1
1 mg
$139.00
In stock
1338-5
5 mg
$395.00
In stock
1338-25
25 mg
$1,295.00
In stock
1338-100
100 mg
$2,795.00
In stock

AFDye™ 488 NHS Ester (Alexa Fluor® 488 NHS Ester eqiuivalent) is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Abs/Em Maxima
494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight
631.54 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 488 NHS Ester (Alexa Fluor® 488 NHS Ester equivalent) is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AFDye™ ideally suited for the 488 nm line. AFDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AFDye™ 488 NHS Ester can be used a less expensive alternative to Alexa Fluor® 488 NHS Ester.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

AFDye488

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