AZDye 488 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1338-1
1 mg
$139.00
In stock
1338-5
5 mg
$330.00
In stock
1338-25
25 mg
$1,395.00
In stock
1338-100
100 mg
$2,250.00
In stock

    AZDye™ 488 NHS Ester is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

    AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AZDye™ 488 NHS Ester can be used an alternative to Alexa Fluor® 488 NHS Ester.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    494/517 nm
    Extinction Coefficient
    73,000
    Flow Cytometry Laser Line
    488 nm
    Microscopy Laser Line
    488 nm
    Spectrally Similar Dyes
    FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
    Molecular weight
    631.54 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Orange to light red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye™ 488 NHS Ester  is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

    AZDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AZDye™ ideally suited for the 488 nm line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

    AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AZDye™ 488 NHS Ester can be used a less expensive alternative to Alexa Fluor® 488 NHS Ester.

    DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

    AZDye488

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