AZDye 488 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1520-1
1 mg
$159.00
In stock
1520-5
5 mg
$495.00
In stock
1520-25
25 mg
$1,495.00
In stock
1520-100
100 mg
$2,795.00
In stock

    AZDye™ 488 Maleimide is a green-fluorescent, water-soluble dye with pH insensitive emission from pH 4 to pH 10. This dye is ideally suited to the 488 nm laser line. AZDye 488 is one of the brightest dyes commercially available with fluorescence quantum yield close to 100%.

    Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to either a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

    AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AZDye™ 488 NHS Ester can be used an alternative to Alexa Fluor® 488 C5 Maleimide

    Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

    Abs/Em Maxima
    494/517 nm
    Extinction Coefficient
    73,000
    Flow Cytometry Laser Line
    488 nm
    Microscopy Laser Line
    488 nm
    Spectrally Similar Dyes
    FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
    Molecular weight
    698.67 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Orange to light red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye488

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