AFDye 488 DBCO

1278-1

1 mg

$149.00

In stock

1278-5

5 mg

$475.00

In stock

1278-25

25 mg

$1,495.00

In stock

AFDye™ 488 DBCO (Alexa Fluor® 488 DIBO analog) is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AFDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Alexa Fluor® 488 is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima

494/517 nm

Extinction Coefficient

73,000

Flow Cytometry Laser Line

488 nm

Microscopy Laser Line

488 nm

CF260

0.35

CF280

0.19

Spectrally Similar Dyes

FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488

Molecular weight

792.12 (protonated)

CAS

N/A

Solubility

Water, DMSO, DMF

Appearance

Orange to light red solid

Storage conditions

-20°C. Desiccate

Shipping conditions

Ambient temperature

AFDye488

Simon P. Wisnovsky, et al. (2020). Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. PNAS, 117 (41), 25293-25301. [PNAS]
Loebel, C., et al. (2020). Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte–Hydrogel Interface. Adv. Funct. Mater. [PubMed]
Loebel, C., et al. (2019). Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels. Nature Materials, 18, 883-891. [PubMed]

CoA Lot 2232