AFDye 488 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1278-1
1 mg
$139.00
In stock
1278-5
5 mg
$445.00
In stock
1278-25
25 mg
$1,395.00
In stock

AFDye™ 488 DBCO (Alexa Fluor® 488 DIBO analog) is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AFDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Alexa Fluor® 488 is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight
792.12 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 488 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AFDye™ 488 DBCO can be used a less expensive alternative to Alexa Fluor® 488 sDIBO

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

AFDye488

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