AFDye 488 DBCO
AFDye™ 488 DBCO (Alexa Fluor® 488 DIBO analog) is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AFDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Alexa Fluor® 488 is a registered trademark of Thermo Fisher Scientific.
AFDye™ 488 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AFDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AFDye™ 488 DBCO can be used a less expensive alternative to Alexa Fluor® 488 sDIBO