AFDye 488 Cadaverine

AFDye™ 488 Cadaverine (Alexa Fluor® 488 Cadeverine equivalent) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.

AFDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AFDye™ ideally suited for the 488 nm line. AFDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AFDye™ 488 Cadaverine can be used a less expensive alternative to Alexa Fluor® 488 Cadaverine.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.