AZDye 488 Biotin

Catalog#
Unit Size
Price (USD)
Availability
Qty
1395-1
1 mg
$179.00
In stock
1395-5
5 mg
$595.00
In stock

    AZDye 488 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission.

    AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488 and CF® 488 Dye.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    494/517 nm
    Extinction Coefficient
    73,000
    Flow Cytometry Laser Line
    488 nm
    Microscopy Laser Line
    488 nm
    Spectrally Similar Dyes
    FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
    Molecular weight
    935.00 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Orange to light red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye™ 488 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye™ 488 Biotin is a water soluble reagent and its green fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

    AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

    AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A.

    DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

    AZDye488

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