AZDye 488 Azide
AZDye™ 488 Azide is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488 and CF® 488 Dye.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.
- Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms, 9, 1578. [MDPI]
- Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]
- Baskin, J. A., et al. (2021). A chemoproteomics approach to profile phospholipase D-derived phosphatidyl alcohol interactions. Cambridge: Cambridge Open Engage, This content is a preprint and has not been peer-reviewed. [ChemRxiv.]
An alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media.