AFDye 488 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1277-1
1 mg
$139.00
In stock
1277-5
5 mg
$395.00
In stock
1277-25
25 mg
$1,295.00
In stock

AFDye™ 488 Alkyne (Alexa Fluor® 488 Alkyne equivalent) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AFDye™ 488 DBCO) is also available for application where the presence of copper is not acceptable.

Alexa Fluor® is a registered trademark of ThermoFisher Scientific.

Abs/Em Maxima
494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight
773.91 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 488 Alkyne (Alexa Fluor® 488 Alkyne equivalent) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AFDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. AFDye™ 488 Alkyne can be used a less expensive alternative to Alexa Fluor® 488 Alkyne.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

AFDye488

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Excitation maximum: 555 nm
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