AFDye 430 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1274-1
1 mg
$129.00
In stock
1274-5
5 mg
$395.00
In stock
1274-25
25 mg
$1,295.00
In stock

AFDye™ 430 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Abs/Em Maxima
430/537 nm
Extinction Coefficient
15,000
Spectrally Similar Dyes
Alexa Fluor® 430, CF® 430
Molecular weight
761.81
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 430 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.

AFDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.

AFDye™ 430 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

Alexa Fluor® Dye is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye430

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