AZDye 430 Alkyne
AZDye™ 430 Alkyne is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AZDye™ 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
A probe for copper-less azide detection (AZDye™ 430 DBCO) is also available for application where the presence of copper is not acceptable.
AZDye™ 430 Alkyne is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AZDye™ 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.
AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.
For application where the presence of copper is not acceptable, please consider our AZDye™ 430 DBCO probe for copper-less detection of azide-modified molecules.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
Excitation maximum: 590 nm
Emission maximum: 617 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm