AFDye 430 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1273-1
1 mg
$129.00
In stock
1273-5
5 mg
$395.00
In stock
1273-25
25 mg
$1,295.00
In stock

AFDye™ 430 Alkyne (Alexa Fluor® 430 Alkyne equivalent) is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AFDye™ 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AFDye™ 430 DBCO) is also available for application where the presence of copper is not acceptable.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
430/537 nm
Extinction Coefficient
15,000
Spectrally Similar Dyes
Alexa Fluor® 430, CF® 430
Molecular weight
540.55
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 430 Alkyne (Alexa Fluor® 430 Alkyne equivalent) is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AFDye™ 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AFDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.

AFDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.

For application where the presence of copper is not acceptable, please consider our AFDye™ 430 DBCO probe for copper-less detection of azide-modified molecules.

Alexa Fluor® Dye is a registered trademark of Invitrogen, part of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye430

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