AFDye 405 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1308-1
1 mg
$149.00
In stock
1308-5
5 mg
$445.00
In stock
1308-25
25 mg
$1,395.00
In stock

    AFDye™ 405 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Abs/Em Maxima
    402/424 nm
    Extinction Coefficient
    35,000
    Flow Cytometry Laser Line
    405 nm
    Microscopy Laser Line
    405 nm
    Spectrally Similar Dyes
    Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
    Molecular weight
    718.68 (protonated) 1022.26 (triethylammonium salt)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Yellow solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 405 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    AFDye™ 405 is structurally similar and spectrally is almost identical to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, or any other sulfonated pyrene based fluorescent dyes.

    DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    AFDye405

    1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
    2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
    3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
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