AZDye 405 NHS Ester
AZDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy. Its excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.
AZDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy. Its excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.
DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
Excitation maximum: 648 nm Emission maximum: 671 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm
Excitation maximum: 590 nm Emission maximum: 617 nm