AFDye 405 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1337-1
1 mg
$129.00
In stock
1337-5
5 mg
$395.00
In stock
1337-25
25 mg
$1,095.00
In stock
1337-100
100 mg
$1,895.00
In stock

AFDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy. Its excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

Abs/Em Maxima
402/424 nm
Extinction Coefficient
35,000
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Spectrally Similar Dyes
Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight
726.70 (protonated) 1030.27 (triethylammonium salt)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that often used in multicolor applications, including flow cytometry and super-resolution microscopy. Its excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AFDye™ 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

AFDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye405

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