AFDye 405 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1307-1
1 mg
$129.00
In stock
1307-5
5 mg
$395.00
In stock
1307-25
25 mg
$1,295.00
In stock

AFDye™ 405 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

Abs/Em Maxima
402/424 nm
Extinction Coefficient
35,000
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Spectrally Similar Dyes
Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight
640.65 (protonated) 706.60 (sodium salt)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AFDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AFDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye405

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