AFDye 405 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1307-1
1 mg
$139.00
In stock
1307-5
5 mg
$445.00
In stock
1307-25
25 mg
$1,395.00
In stock

    AFDye™ 405 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AFDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

    Abs/Em Maxima
    402/424 nm
    Extinction Coefficient
    35,000
    Flow Cytometry Laser Line
    405 nm
    Microscopy Laser Line
    405 nm
    Spectrally Similar Dyes
    Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
    Molecular weight
    640.65 (protonated) 706.60 (sodium salt)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Yellow solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

    AFDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

    AFDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

    DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    AFDye405

    Related Products
    AFDye 594 DBCO

    Excitation maximum: 590 nm
    Emission maximum: 617 nm

    AFDye 647 DBCO

    Excitation maximum: 648 nm
    Emission maximum: 671 nm

    AFDye 488 DBCO

    Excitation maximum: 494 nm
    Emission maximum: 517 nm