AFDye 350 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1268-1
1 mg
$149.00
In stock
1268-5
5 mg
$445.00
In stock
1268-25
25 mg
$1,395.00
In stock

This products is temporarily unavailable.

Abs/Em Maxima
346/445 nm
Extinction Coefficient
19,000
Spectrally Similar Dyes
Alexa Fluor® 350, CF® 350, DyLight 350, AMCA
Molecular weight
515.12
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Grey to yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 350 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AFDye™ 350 (Alexa Fluor® 350 equivalent) is a water-soluble moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AFDye350

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

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