AZDye 350 NHS Ester
AZDye™ 350 NHS Ester is an amine reactive, water-soluble blue emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
AZDye™ 350 NHS Ester is an amine reactive, water-soluble blue emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
AZDye™ 350 is a water-soluble moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.
Excitation maximum: 590 nm
Emission maximum: 617 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm
