AFDye 350 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1336-1
1 mg
$95.00
In stock
1336-5
5 mg
$295.00
In stock
1336-25
25 mg
$895.00
In stock
1336-100
100 mg
$2,095.00
In stock

AFDye™ 350 NHS Ester (Alexa Fluor 350 NHS Ester equivalent) is an amine reactive, water-soluble blue emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
346/445 nm
Extinction Coefficient
19,000
Spectrally Similar Dyes
Alexa Fluor® 350, CF® 350, DyLight 350, AMCA
Molecular weight
410.35
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Grey to yellow solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 350 NHS Ester (Alexa Fluor 350 NHS Ester equivalent) is an amine reactive, water-soluble blue emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AFDye™ 350 is a water-soluble moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AFDye 350 NHS Ester is NOT manufactures, distributed, or affiliated in any way with Thermo Fisher Scientific.

AFDye350

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