AFDye 488 TCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1356-1
1 mg
$139.00
In stock
1356-5
5 mg
$495.00
In stock
1356-25
25 mg
$1,295.00
In stock

AFDye™ 488 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M-1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

Abs/Em Maxima
494/517 nm
Extinction Coefficient
73,000
Spectrally Similar Dyes
Alexa Fluor® 488, Atto™ 488, CF® 488 Dye, DyLight® 488, FITC
Molecular weight
770.82
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Orange solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 488 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

AFDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AFDye™ 488 is NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.

AFDye488

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Excitation maximum: 494 nm
Emission maximum: 517 nm