AFDye 488 TCO
AFDye™ 488 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
AFDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.
DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AFDye™ 488 is NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.
|Abs/Em Maxima||494/517 nm|
|Spectrally similar dyes||Alexa Fluor® 488, Atto™ 488, CF™ 488 Dye, DyLight™488, FITC|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|
|TCO-NHS Ester||An amine-reactive, succinimidyl carbonate/NHS functionalized cyclooctene building block.|