AFDye 488 Tetrazine
A bright, green-fluorescent probe used for detection TCO-tagged biopolymers. AFDye 488 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must AFDye 488 Tetrazine probe would of great value.
Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.
AFDye™ 488 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 493 and 517 nm, respectively. It can be used with the 488 nm laser line. AFDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.
DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AFDye™ 488 is NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.
An amine-reactive, succinimidyl carbonate/NHS functionalized cyclooctene building block.
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A tetrazine-activated Cy5 dye.
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