AFDye 488 Tetrazine

Catalog#
Unit Size
Price (USD)
Availability
Qty
1361-1
1 mg
$159.00
In stock
1361-5
5 mg
$695.00
In stock
1361-25
25 mg
$2,150.00
In stock

A bright, green-fluorescent probe used for detection TCO-tagged biopolymers. AFDye 488 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must AFDye 488 Tetrazine probe would of great value.

Abs/Em Maxima
493/517 nm
Extinction Coefficient
83,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
Alexa Fluor® 488, Atto™ 488, CF™ 488 Dye, DyLight™488
Molecular weight
703.66
CAS
N/A
Solubility
Water, MeOH, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

AFDye™ 488 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 493 and 517 nm, respectively. It can be used with the 488 nm laser line. AFDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AFDye™ 488 is NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.

AFDye488

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