Metabolic labeling of glycans with unnatural sialic acid analogues has emerged as an invaluable tool for studying sialic acid biology. It has been showed that 9-azido-9-deoxy-N-acetylneuraminic acid (9AzNeu5Ac), a Neu5Ac analogue with the substitution of 9-hydroxyl group with an azide, could be metabolized and incorporated into sialoglycans in living cells and mice. The incorporated sialic acid analogue, 9AzNeu5Ac, enable visualization the cell-surface sialoglycans and glycoproteomic profiling by click-labeling with fluorophores and affinity tags, respectively.
- Cheng, B., et al. (2019). 9-Azido Analogues of Three Sialic Acid Forms for Metabolic Remodeling of Cell-Surface Sialoglycans. ACS Chem Biol. 14(10), 2141-7 [PubMed]
- Zhang, C., et al. (2016). In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy. Proc Natl Acad Sci U S A., 113 (19), 5173-8. [PubMed]
- Xie, R., et al. (2012). Cell-selective metabolic glycan labeling based on ligand-targeted liposomes. J. Am. Chem. Soc., 134(24), 9914-7. [ACSPublications]
Azide modified, non-mammalian disaccharide trehalose precursor for essential cell wall glycolipids and other metabolites.