5-TAMRA NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1243-25
25 mg
$95.00
In stock
1243-100
100 mg
$245.00
In stock
1243-1000
1000 mg
$1,095.00
In stock

5-TAMRA NHS Ester (5-Carboxytetramethylrhodamine) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. This dye is supplied purified single isomer 5. 5-TAMRA has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

Abs/Em Maxima
553/575 nm
Extinction Coefficient
92,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes
Alexa Fluor® 555, CF® 555, DyLight® 549
Molecular weight
527.53
CAS
150810-68-7
Solubility
Water, DMSO, DMF
Purity
>90% (HPLC)
Appearance
Dark red amorphous solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

TAMRA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

TAMRA

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