5-TAMRA Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1255-1
1 mg
$75.00
In stock
1255-5
5 mg
$139.00
In stock
1255-25
25 mg
$395.00
In stock
1255-100
100 mg
$795.00
In stock

    5-TAMRA Alkyne (also known as TAMRA Alkyne, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal azide-tagged biomolecules via a copper-catalyzed click reaction (CuAAC).

    Abs/Em Maxima
    553/575 nm
    Extinction Coefficient
    89,000
    Spectrally Similar Dyes
    Alexa Fluor® 546, Atto™ 543, CF® 543 Dye
    Molecular weight
    467.52
    CAS
    N/A
    Solubility
    DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Dark red amorphous solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    5-TAMRA Alkyne (also known as TAMRA Alkyne, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

    Although the mixed isomers of 5(6)-TAMRA Alkyne is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

    5-TAMRA Alkyne is structurally identical to Tetramethylrhodamine (TAMRA) Alkyne (5-Carboxytetramethylrhodamine, Propargylamide), 5-isomer ,Catalog number: T10183, sold by Thermo Fisher Scientific and can be used as cost effective replacement.

    TAMRA

    1. Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]
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