5-Ethynyl Uridine (5-EU)
5-Ethynyl uridine (5-EU) is a metabolic labeling reagent used to detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide using two-step technique.
In step one, the alkyne-containing uridine analog 5-EU is fed to cells or animals and actively incorporated into RNA. In step two the incorporated 5-EU is detected via chemoselective ligation or “click” reaction between an azide and an alkyne where the modified biomolecules are detected with a corresponding azide-containing dye or hapten. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).
|Solubility||Water, DMSO, DMF|
|Appearance||Off white to grey crystalline|
|Shipping conditions||Ambient temperature|
|Fluorescent Picolyl Azides||Next generation fluorescent azide probes for high sensitivity detection.|
|Fluorescent Labeling Kits||All-inclusive kits to perform the copper-catalyzed fluorescent labeling.|
|Auxiliary Reagents||Stand-alone reagents, and complete kits that contain all necessary reagents to perform the copper-catalyzed click reaction.|