Click-&-Go Protein Enrichment Kits
Click-&-GoTM Protein Enrichment Kits is an efficient, biotin-streptavidin free tool for capture of alkyne or azide-modified proteins on an azide or alkyne-agarose resin. Covalent, biotin-streptavidin free capture of modified proteins possess many advantages over to biotin or lectin based enrichment approaches, including universal differential profiling of expressed and post-translationally modified (PTM) proteins, improved signal-to-noise ratio, increased selectivity, and improved the detection of low abundant proteins.
Alkyne or azide-modified proteins, or their post-translationally modified forms, are efficiently captured from complex protein extracts on the azide or alkyne-agarose resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.
|Click-&-Go Plus Protein Enrichment Kit for Capture of Alkyne-tagged Proteins||Protein Enrichment Kit for covalent capture of alkyne-tagged proteins.|
|Click-&-Go Protein Enrichment Kit for Capture of Alkyne-tagged Proteins||Protein Enrichment Kit for covalent capture of alkyne-tagged proteins.|
|Click-&-Go Protein Enrichment Kit for Capture of Azido-tagged Proteins||Protein Enrichment Kit for covalent capture of azido-tagged proteins.|